LCT -13910C>T RealFast® Assay

The LCT -13910C>T RealFast™ Assay is a fast and accurate real-time PCR test for the detection of the LCT -13910C>T variant in the upstream regulatory region of the lactase (LCT) gene. The kit is designed to identify patients with the LCT -13910CC genotype, which causes lactase non-persistence and thus intolerance to dietary lactose. The qualitative assay discriminates the three possible LCT -13910C>T genotypes in a human DNA extract: CC (non-persistent),

• Kits are based on reverse-hybridization of biotinylated PCR products.
• Minimal equipment required (thermal cycler, shaking water bath).
• Technology combines probes for mutations and controls in a parallel array of allele-specific oligonucleotides.
• Functions using immobilized oligos on a teststrip.
• Strips show mutations by enzymatic color reaction visible to the naked eye.
• Proprietary software package (Evaluator™) that aids in data interpretation, storage, and archiving.

Publications

Lactose Maldigestion, Malabsorption, and Intolerance: A Comprehensive Review with a Focus on Current Management and Future Perspectives (F. Fassio, 2018)

Lactose Intolerance in Adults: Biological Mechanism and Dietary Management (Y. Deng, 2002)

Identification of a variant associated with adult-type hypolactasia (N. Enattah, 2002)

Allelic Variants Detected

LCT -13910C>T