The ViennaLab RealFast™ Assays are based on real-time PCR and hydrolysis probes, also commonly called TaqMan® probes.
The sequence-specific oligonucleotide probes carry a fluorescent reporter dye at the 5´-end and a quencher dye at the 3´-end. While the probe is intact, the quencher is close enough to the reporter to suppress the fluorescent signal of the 5´-fluorophore.
During the combined annealing/extension phase of PCR, the probe is cleaved by the 5´ to 3´ exonuclease activity of Taq DNA polymerase, thereby separating the fluorophore from the quencher dye.This process results in detectable fluorescence, which is proportional to the amount of accumulated PCR product.
Single Marker Detection
| Area of Interest Carbohydrate Intolerance Cardiovascular Disease Genetic Predisposition Pharmacogenetics
| Assay Name FXII 46C>T FXIII V34L MTHFR 677C>T MTHFR 1298A>C PAI-1 4G/5G PTH 20210G>A HLA-B27 HLA-B5701 IL28B SLCO1B1c.521T>C VKORC1 -1639G>A | Catalog Number 7-240 / 7-243 7-250 / 7-253 7-160 / 7-163 7-170 / 7-173 7-180 / 7-183 7-120 / 7-123 7-620 / 7-623 7-610 / 7-613 7-200 / 7-203 7-210 /-7-213 7-190 / 7-193 7-640 / 7-643 7-630/ 7-633 |
Multiplex RealFast™ Assays
| Area of Interest
Cardiovascular Disease COPD / AAT deficiency Pharmacogenetics | Assay Name
FV-PTH mpx MTHFR mpx AAT mpx CYP2C9 mpx | Catalog Number
7-115 / 7-118 7-165 / 7-168 7-265 / 7-268 7-225 / 7-228 |
QuickGuide RealFast™ Genotyping for AB 7500 Fast
QuickGuide RealFast™ Genotyping for BioRad CFX96
QuickGuide RealFast™ Genotyping for Agilent Mx3005P™
QuickGuide RealFast™ Genotyping for LightCycler® 480II
QuickGuide RealFast™ Genotyping for Rotor-Gene® 6000
