KRAS – BRAF StripAssay®

The KRAS – BRAF StripAssay® is a combination assay designed to detect 10 mutations in codons 12 and 13 in the KRAS gene as will as the V600E mutation in BRAF gene. The kit offers a highly cost effective option for dual KRAS-BRAF testing.

KRAS – BRAF StripAssay® Brochure

  • Kits are based on reverse-hybridization of biotinylated PCR products.
  • Minimal equipment required (thermal cycler, shaking water bath).
  • Technology combines probes for mutations and controls in a parallel array of allele-specific oligonucleotides.
  • Functions using immobilized oligos on a teststrip.
  • Strips show mutations by enzymatic color reaction visible to the naked eye.
  • Proprietary software package (Evaluator™) that aids in data interpretation, storage, and archiving.

 

Publications

457 Assessment of BRAF, NRAS, KRAS and EGFR mutation status in thyroid nodules (Novy 2015)

Comparison of neuroendocrine differentiation and KRAS/NRAS/BRAF/PIK3CA/TP53 mutation status in primary and metastatic colorectal cancer (Kleist 2014)

BRAF mutation status in primary and metastatic melanomas in two regions with differing potential ultraviolet radiation exposure (Massad 2014)

BRAF Analysis on a Spectrum of Melanocytic Neoplasms: An Epidemiological Study Across Differing UV Regions (Saroufim 2013)

BRAF mutational epidemiology in dysplastic nevi: does different solar UV radiation exposure matter? (Saroufim 2013)

Duplex Reverse-Hybridization Assay for The Simultaneous Detection of KRAS/BRAF Mutations in FFPE-extracted Genomic DNA from Colorectal Cancer Specimens (2013 Buxhofer-Ausch)

Mutations and Deletions Detected

KRAS-BRAF StripAssay® Catalog 5-580

codon 12: Ala, Arg, Asp, Cys, Ile, Leu, Ser, Val

codon 13: Asp, Cys

V600E (Val > Glu)

 

For Research Use Only [RUO]. Not for use in diagnostic procedures.