BRAF StripAssay®

In as many as 8% of human tumors there is an occurrence of mutations in the BRAF gene. The BRAF StripAssay® is one of two efficient and cost effective StripAssay® solutions to detect this mutation when in rapid fashion.

• Kits are based on reverse-hybridization of biotinylated PCR products.
• Minimal equipment required (thermal cycler, shaking water bath).
• Technology combines probes for mutations and controls in a parallel array of allele-specific oligonucleotides.
• Functions using immobilized oligos on a teststrip.
• Strips show mutations by enzymatic color reaction visible to the naked eye.
• Proprietary software package (Evaluator™) that aids in data interpretation, storage, and archiving.

Publications

457 Assessment of BRAF, NRAS, KRAS and EGFR mutation status in thyroid nodules (Novy 2015)

Comparing BRAF mutation status in matched primary and metastatic cutaneous melanomas: Implications on optimized targeted therapy (Saroufim 2014)

Comparison of neuroendocrine differentiation and KRAS/NRAS/BRAF/PIK3CA/TP53 mutation status in primary and metastatic colorectal cancer (Kleist 2014)

BRAF mutation status in primary and metastatic melanomas in two regions with differing potential ultraviolet radiation exposure (Massad 2014)

BRAF Analysis on a Spectrum of Melanocytic Neoplasms: An Epidemiological Study Across Differing UV Regions (Saroufim 2013)

BRAF mutational epidemiology in dysplastic nevi: does different solar UV radiation exposure matter? (Saroufim 2013)

Mutations Detected

BRAF 600/601 StripAssay® Catalog 5-560

V600A (c.1799T>C),

V600D (c.1799_1800TG>AT)

V600E (c.1799T>A)

V600E (c.1799_1800TG>AA),

V600G (c.1799T>G)

V600K (c.1798_1799GT>AA)

V600M (c.1798G>A)

V600R (c.1798_1799GT>AG)

K601E (c.1801A>G)

BRAF StripAssay® Catalog 5-570

V600E (c.1799T>A)