MolYsis™ Plus kits

MolYsis™ is a tool for the removal of human DNA and enrichment of bacteria and fungi from liquid samples.

A major problem with blood culture PCR is the contamination of DNA preparations with PCR-inhibitors like polyanetholesulfonate (SPS). MolYsis™ Plus is a special solution for the extraction of pure bacterial and fungal DNA from positive blood cultures (0.2 ml). The removal of PCR inhibitors and human DNA allows the reliable PCR amplification of microbial DNA for identification by sequencing and other analyses.

MolYsis™ Kits are effective for DNA with a range of samples including:

Documentation

MolYsis™ Brochure

Catalog Numbers

Publications

Detection and quantification of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Streptococcus oralis in blood samples with different microbiological identification methods: An in vitro study (Marin M. 2017)

Response to antiseptic agents of periodontal pathogens in in vitro biofilms on titanium and zirconium surfaces (Sánchez M., 2017)

Messages from the first International Conference on Clinical Metagenomics (Etienne R. 2017)

Antibiotic resistance potential of the healthy preterm infant gut microbiome (Graham, R 2017)

Progress in the molecular diagnosis of Lyme disease (Ružić-Sabljić E. 2017)

Broad-spectrum molecular detection of fungal nucleic acids by PCR-based amplification techniques. In: Lion T (ed.), Human Fungal Pathogen Identification: Methods and Protocols, Methods in Molecular Biology (Lion T, 2017)

Same-day diagnostic and surveillance data for tuberculosis via whole genome sequencing of direct respiratory samples (Votintseva A, 2017)

A rapid, highly sensitive and culture-free detection of pathogens from blood by positive enrichment (Vutukuru M, 2016)

Evaluation of a commercial multiplex PCR assay for detection of pathogen DNA in blood from patients with suspected sepsis (Ziegler I, 2016)

Metagenomic analysis of bloodstream infections in patients with acute leukemia and therapy-induced neutropenia (Gyarmati P, 2016)

Comparison of the detection of periodontal pathogens in bacteraemia after tooth brushing by culture and molecular techniques (Marin M, 2016)

Comparison of microbial DNA enrichment tools for metagenomic whole genome sequencing (Thoendel M, 2016)

A study of the infant nasal microbiome development over the first year of life and in relation to their primary adult caregivers using cpn60 universal target (UT) as a phylogenetic marker (Peterson S, 2016)

Mycobacterium avium subspecies paratuberculosis infection modifies gut microbiota under different dietary conditions in a rabbit model (Arrazuria R, 2016)

Direct screening of blood by PCR and pyrosequencing for a 16S rRNA gene target from emergency department and intensive care unit patients being evaluated for bloodstream infection (Moore M, 2016)

Higher blood volumes improve the sensitivity of direct PCR diagnosis of blood stream tuberculosis among HIV-positive patients: an observation study (Bwanga F, 2015)