Oasis Diagnostics offers reagents ranging from DNA-free Taq polymerase, MolTaq 16S and DNA-free Mastermix 16S kits. Mastermix 16S Primer and Mastermix 16S Complete for the detecting bacteria by the amplifying a highly variable region of the 16S rRNA gene.
The Mastermix 16S Basic and Mastermix 16S Dye kits are amenable to combination with your own primers, these kits contain all other components required for running the PCR save for the primers.
One example of how to identify bacteria is to take the sequenced amplicons and combine them with online homology BLAST search. Using the Molzym primers will see them bound to specified regions within the amplified DNA (approx. 460 bp), this provides highly discriminative sequences for strain identification.
|Mastermix 16S Complete (100 Reactions)
Mastermix 16S Complete (250 Reactions)
Mastermix 16S Complete (1000 Reactions)
Mastermix 16S Primer for eubacterial PCR assay
Mastermix 16S/18S Dye (100 Reactions)
Mastermix 16S/18S Dye (250 Reactions)
Mastermix 16S/18S Dye (1000 Reactions)
Mastermix 16S/18S Basic (100 Reactions)
Mastermix 16S/18S Basic (250 Reactions)
Mastermix 16S/18S Basic (1000 Reactions)
Mastermix 18S Complete (100 Reactions)
Mastermix 18S Complete (250 Reactions)
Mastermix 18S Complete (1000 Reactions)
Thermostable DNA Polymerase – MolTaq 16S/18S (100 Reactions)
Thermostable DNA Polymerase – MolTaq 16S/18S (500 Reactions)
Thermostable DNA Polymerase – MolTaq 16S/18S (1000 Reactions)
DNA-free water, PCR grade
DNA-free Water – Positive Control DNA (P1)
Sequencing Primer – Set of Eubacterial Sequencing Primers (SeqGN16 & SeqGP16)
Service evaluation to establish the sensitivity, specificity and additional value of broad-range 16S rDNA PCR for the diagnosis of infective endocarditis from resected endocardial material in patients from eight UK and Ireland hospitals (Harris K, 2014)
Effect of supplementing varying inclusion levels of laminarin and fucoidan on growth performance, digestibility of diet components, selected faecal microbial populations and volatile fatty acid concentrations in weaned pigs (Walsh A, 2013)