ViennaLab RealFast™ Assays

The ViennaLab RealFast™ Assays are based on real-time PCR and hydrolysis probes, also commonly called TaqMan® probes.

The sequence-specific oligonucleotide probes carry a fluorescent reporter dye at the 5´-end and a quencher dye at the 3´-end. While the probe is intact, the quencher is close enough to the reporter to suppress the fluorescent signal of the 5´-fluorophore.

During the combined annealing/extension phase of PCR, the probe is cleaved by the 5´ to 3´ exonuclease activity of Taq DNA polymerase, thereby separating the fluorophore from the quencher dye.This process results in detectable fluorescence, which is proportional to the amount of accumulated PCR product.

 

Quick Guides for Running RealFast™ Assays

 
QuickGuide RealFast™ Genotyping for AB 7500 Fast
 
QuickGuide RealFast™ Genotyping for BioRad CFX96
 
QuickGuide RealFast™ Genotyping for Agilent Mx3005P™
 
QuickGuide RealFast™ Genotyping for LightCycler® 480II
 
QuickGuide RealFast™ Genotyping for Rotor-Gene® 6000

For Research Use Only [RUO]. Not for use in diagnostic procedures.