ViennaLab RealFast™ Assays

The ViennaLab RealFast™ Assays are based on real-time PCR and hydrolysis probes, also commonly called TaqMan® probes.

The sequence-specific oligonucleotide probes carry a fluorescent reporter dye at the 5´-end and a quencher dye at the 3´-end. While the probe is intact, the quencher is close enough to the reporter to suppress the fluorescent signal of the 5´-fluorophore.

During the combined annealing/extension phase of PCR, the probe is cleaved by the 5´ to 3´ exonuclease activity of Taq DNA polymerase, thereby separating the fluorophore from the quencher dye.This process results in detectable fluorescence, which is proportional to the amount of accumulated PCR product.

Catalog of Available RealFast™ Assays

 

Single Marker Detection

Area of Interest

Carbohydrate Intolerance

Cardiovascular Disease

 

 

 

 

 

 

 

Genetic Predisposition

Pharmacogenetics

 

 

 

Assay Name

LCT -13910C>T

FGB -455G>A

FV Leiden

FXII 46C>T

FXIII V34L

MTHFR 677C>T

MTHFR 1298A>C

PAI-1 4G/5G

PTH 20210G>A

HLA-B27

HLA-B5701

IL28B

SLCO1B1c.521T>C

VKORC1 -1639G>A

Catalog Number

7-150 / 7-153

7-230 / 7-233

7-110 / 7-113

7-240 / 7-243

7-250 / 7-253

7-160 / 7-163

7-170 / 7-173

7-180 / 7-183

7-120 / 7-123

7-620 / 7-623

7-610 / 7-613

7-200 / 7-203

7-210 /-7-213

7-190 / 7-193

Multiplex RealFast™ Assays

Area of Interest

Cardiovascular Disease

 

COPD / AAT deficiency

Pharmacogenetics

Assay Name

FV-PTH mpx

MTHFR mpx

AAT mpx

CYP2C9 mpx

Catalog Number

7-115 / 7-118

7-165 / 7-168

7-265 / 7-268

7-225 / 7-228

 

Quick Guides for Running RealFast™ Assays

 

QuickGuide RealFast™ Genotyping for AB 7500 Fast

QuickGuide RealFast™ Genotyping for BioRad CFX96

QuickGuide RealFast™ Genotyping for Agilent Mx3005P™

QuickGuide RealFast™ Genotyping for LightCycler® 480II

QuickGuide RealFast™ Genotyping for Rotor-Gene® 6000

Additional Documentation

 

2018 RealFast™ Assay Handout

For Research Use Only [RUO]. Not for use in diagnostic procedures.