MolYsis™ is a tool for the removal of human DNA and enrichment of bacteria and fungi from liquid samples.
A major problem with blood culture PCR is the contamination of DNA preparations with PCR-inhibitors like polyanetholesulfonate (SPS). MolYsis™ Plus is a special solution for the extraction of pure bacterial and fungal DNA from positive blood cultures (0.2 ml). The removal of PCR inhibitors and human DNA allows the reliable PCR amplification of microbial DNA for identification by sequencing and other analyses.
MolYsis™ Kits are effective for DNA with a range of samples including:
|MolYsis ™ Plus (50 Reactions)
MolYsis ™ Plus (100 Reactions)
Detection and quantification of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Streptococcus oralis in blood samples with different microbiological identification methods: An in vitro study (Marin M. 2017)
Broad-spectrum molecular detection of fungal nucleic acids by PCR-based amplification techniques. In: Lion T (ed.), Human Fungal Pathogen Identification: Methods and Protocols, Methods in Molecular Biology (Lion T, 2017)
A study of the infant nasal microbiome development over the first year of life and in relation to their primary adult caregivers using cpn60 universal target (UT) as a phylogenetic marker (Peterson S, 2016)