High PapillomaStrip

High PapillomaStrip kit is based on the principle of reverse hybridization and allows for the detection and identification of 19 medium-high risk genital HPVs in DNA samples from cervico-uterine smears or biopsies.

Specific primers for E6-E7 regions for each of the 37 HPVs detected with the kit are used to perform the multiplex PCR. Since the expression of the E6-E7 genes is directly associated with carcinogenesis, detection of these targets may provide significant biological and pathological information.

PapillomaStrip-High Risk Product Insert

• Kits are based on reverse-hybridization of PCR products.
• All PCR reagents are included in the kit: Taq polymerase, primers and Master Mix.
• Several kinds of methods have been validated for DNA extraction: salting-out, silica columns, commercial kits (automated extraction), etc.
• Strips show high risk HPV subtypes by enzymatic color reaction visible to the naked eye.
• Minimal equipment required (thermal cycler).
• Automated protocols available for Auto-LiPA / Profiblot and AUTOBLOT instruments.

HPV subtypes Detected

19 human Papillomavirus subtypes of medium-high risk: 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 69, 73 and 82.